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Chapters Included in LEXICON:

Chapter #1 - Introduction to Next-Generation RNA Sequencing
Chapter #2 - Next Generation Sequencing: How “Sequencing by Synthesis” Works
Chapter #3 - Experimental and Data Analysis Planning for RNA Sequencing
Chapter #4 - RNA Extraction and Quality Control
Chapter #5 - DNase: To Treat or Not to Treat
Chapter #6 - RNA Pretreatment: Enrichment or Depletion?
Chapter #7 - RNA-Seq Library Preparation: Molecular Biology Basics
Chapter #8 - What are Unique Molecular Identifiers (UMIs) and Why do We Need Them?
Chapter #9 - Indexing Strategies and Solutions
Chapter #10 - Library Preparation Quality Control and Quantification
Chapter #11 - Data Analysis and Quality Control – Primary Analysis
Chapter #12 - Data Analysis and Quality Control – Secondary Analysis
Chapter #13 - Data Analysis and Quality Control – Tertiary Analysis