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Enhance your FFPE RNA-Seq library preps!

Get the best sequencing results for your FFPE samples with CORALL RNA-Seq V2 – Lexogen’s fast and flexible whole transcriptome RNA-Seq Library Prep Kit.

 

 

RNA-Seq from FFPE samples with ease using CORALL RNA-Seq V2!

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Time Saving

CORALL offers a fast and fully automatable whole transcriptome RNA-Seq workflow which is completed in only 4.5 hours.
 

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Challenging material

CORALL’s fragmentation-free protocol and built-in UMIs for RNA-Seq library preparation are ideal for challenging inputs, such as degraded and FFPE RNA.
 

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Low Input Samples

CORALL supports the widest input range (1 ng - 1000 ng) with excellent performance for low input RNA.
 

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Upscale Experiments with Ease

Automation-friendly and fast workflows allow processing of hundreds of samples in a single day and speed up turnover time to results!
 

Are you tired of lengthy and complicated RNA-Seq library preparation for your low input and FFPE samples?

 

Enhance your whole transcriptome sequencing (WTS) workflow and cost-effectiveness for FFPE samples with CORALL RNA-Seq V2 and prepare your samples within one day, regardless of input amount or sample quality!

Fast and flexible library prep in only 4.5 hours ⏱️

In contrast to conventional WTS RNA-Seq, CORALL library preparation requires only 6 steps and can be completed in 4.5 hours (Fig. 1).
CORALL does not require RNA fragmentation and is thus perfectly suited for processing of degraded or FFPE RNA.
 Figure 1: Comparison of CORALL and conventional WTS RNA-Seq library preparation workflows

 Figure 1 | Comparison of CORALL and conventional WTS RNA-Seq library preparation workflows

Excellent performance on FFPE samples

The robust and fragmentation-free workflow including RiboCop rRNA depletion is the ideal choice for degraded
and FFPE RNA (Fig. 2). The sensitive protocol with built-in unique molecular identifiers (UMIs) delivers high-quality RNA-Seq data for all RNA input amounts and qualities.
Figure 2: Gene discovery rates for fresh frozen (FF) and FFPE derived human liver RNA. A) Gene detection rates. The number of detected genes is plotted against the total number of reads mapping uniquely to exons. B) Overlap of detected genes at normalized expression levels >5 CPM (for uniquely mapping reads). 

Figure 2 | Gene discovery rates for fresh frozen (FF) and FFPE derived human liver
RNA. A) Gene detection rates. The number of detected genes is plotted against
the total number of reads mapping uniquely to exons. B) Overlap of detected
genes at normalized expression levels >5 CPM (for uniquely mapping reads). 

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Upscale your experiments with ease!

CORALL RNA-Seq V2 library preparation workflows are fast and automation-friendly. Save time and process hundreds of samples in a single day.

CORALL RNA-Seq V2 Kits

What Our Customers Say

 

“We can support our researchers very well using Lexogen’s CORALL RNA-Seq Library preparation due to its excellent performance on low starting RNA input, combined with unique molecular identifiers and 12 nt unique dual indexes. We find the protocol to be straight-forward and can generate very consistent libraries perfect for standard RNA-Seq projects. In addition, CORALL delivers great results for degraded RNA or FFPE samples with minimal adaptations in very few protocol steps.”
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Dr. Pamela Nicholson
Manager Next Generation Sequencing Platform, University of Bern, Switzerland

CORALL RNA-Seq V2

Lexogen’s fast and flexible library prep kit with exceptional performance for challenging samples

  • The fragmentation-free protocol complete with RiboCop rRNA depletion is the ideal choice for degraded and FFPE RNA.

  • Built-in UMIs for highest data quality especially for challenging RNA qualities and low-quantity samples.

  • CORALL’s time-saving and easy workflow enables processing from sample to sequencing-ready library in less than one day.

  •  CORALL RNA-Seq V2 supports the widest input range (1 ng - 1000 ng) with excellent sensitivity for low input RNA.

Image of the CORALL v2 library prep kit from Lexogen, a set of reagents and protocols for preparing high-quality RNA sequencing libraries The kit enables efficient and reliable library preparation for a wide range of RNA sequencing applications..

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Ordering Information

  • Cat. №  | 171 - 176
    Product Name  CORALL RNA-Seq V2 Library Prep Kit with UDIs (Set A1, A2, A3, A4, B1, or A1- A4)
    Kit Sizes [preps] 24, 96, 384
     
  • Cat. №  | 183 - 184
    Product Name  RiboCop HMR V2 and CORALL Total RNA-Seq V2 Library Prep Kit with UDIs (A1 or B1)    
    Kit Sizes [preps] 24, 96
     

About Lexogen

Lexogen is a biotech company focusing on RNA and complete transcriptome studies using next generation sequencing technologies.

We have been at the forefront of RNA research since 2007, and our technologies and products are being used by thousands of scientists all over the world. “Lexo” in Greek means “word”, and Lexogen stands for the word of genes, which is the transcriptome. We strive to be the pioneers in this fascinating field of RNA science, and together with the fastest-growing technology of the past years, the next generation sequencing (NGS), we are sure that we can make a big contribution to the world.

Contact Us

Lexogen GmbH
Campus Vienna Biocenter 5
1030 Vienna, Austria

Lexogen, Inc.
51 Autumn Pond Park
Greenland, NH 03840, US

Email
info@lexogen.com

sales@lexogen.com

 Telephone:
US +1-603-431-4300
Rest of the world +43 (0) 1 345 1212 

 

Logo Iso certifications 9001 and 13485

Lexogen ISO certifications

We proudly hold both ISO 9001 and ISO 13485 certifications, which not only demonstrate our drive for innovation but also our unwavering devotion to delivering reliable, robust, and cutting-edge solutions to our valued customers.